Why pipette is accurate

The volume delivery and precision differ amongst various types, classes, and, built of graduated pipettes, therefore, creating a space for human error to occur. If high-level accuracy is the concern, then volumetric pipettes are the best choice for reliable results. Welcome to Science Equip!

Why is using a volumetric pipette considered more accurate in a Scienc Home News Why is using a volumetric pipette considered more accurate in a Science-Lab? Leave a comment. Post comment. Recent Posts. Contact Form. Follow us. Contact Us! Tags: why use a pipette , why use a pipette instead of a measuring cylinder , how does a pipette measure. Blog archive. Popular blog tags. The correct pipetting angle and speed, and a smooth, consistent pipetting rhythm contributes to precision.

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Do you want to remove your BOGA from the cart? You can always configure it again from the Campaign Page. What is a cookie? Why do we have a cookies policy? This makes them more accurate for pipetting volatile solvents because there is no place air cushion for the solvent to evaporate.

The lack of air cushion also decreases the chance of contamination when pipetting corrosives and bio-hazardous material, which makes positive displacement pipettes more suitable for working with those reagents. These pipettes are expensive because the barrel and the tip are a unit and both are replaced when pipetting. A cheaper alternative is to use an air displacement pipette with barrier tips, but these only address some of the problems.

This is a rough guide; you made need to have it serviced more often depending on your requirements for accuracy. The service should include re-calibration, greasing of the moving parts, and replacement of any worn-out seals or other parts. If you work in a university or for a large company, there may be pipette clinics you can use. In other cases, you can send your pipettes off to companies that will do it for you. Also, check out our article on how to assess the accuracy of your pipette.

Examine the nose of the barrel where the tip is fitted for any obvious damage. If there is a problem, have it serviced—because it is unlikely to be fit for the job. Storing your pipette this way prevents any liquids that are in the pipette barrel from getting any further inside the pipette and causing corrosion. Any liquid inside the pipette barrel results in contamination at the least and can cause some serious corrosion damage.

Poorly fitting tips allow air to escape when drawing up and dispensing liquid, leading to inaccurate results. The most important rules to follow are:. The mass of the droplet you make should be around 0. Now do the same thing 10 times and record the masses you obtain. When you dispense liquid from your pipette, a coating of liquid is left on the tip, which makes the dispensed volume slightly less than that displayed on the pipette.

Pre-wetting the tip before you pipette will help increase your accuracy. To pre-wet your tip, draw up the liquid into your pipette and then dispense it back into the original vessel. The coating is on the tip. Now, when you draw up the liquid again and dispense it into the receiving vessel, none of it will be left on the tip and lost to wetting.